Cyclin E、p53与PTEN在胃癌组织中的表达及临床意义

摘要

目的 研究细胞周期蛋白E(Cyclin E)、p53、第10号染色体缺失的磷酸酶和张力蛋白同源物基因(PTEN)在胃癌组织中的表达及意义.方法 采用免疫组化法检测65例胃癌和13例胃正常黏膜石蜡标本中Cyclin E、p53及PTEN的表达情况.结果 (1)Cyclin E在胃癌组织中高表达,并随肿瘤浸润深度的加深、淋巴结的转移、临床分期的提高、肿瘤分化程度的降低及出现远处转移而升高(P<0.05).(2)p53在胃癌组织中的高表达,并随肿瘤浸润深度的加深、淋巴结的转移、临床分期的提高、肿瘤分化程度的降低及出现远处转移而升高(P<0.05).(3)PTEN在胃癌组织中低表达,并随肿瘤浸润深度的加深、分化程度的降低、淋巴转移的发生及临床分期的增高及出现远处转移而降低(P<0.05).(4)PTEN的表达与 Cyclin E和p53的表达呈负相关,Cyclin E和p53表达呈正相关(P<0.05).结论 Cyclin E、p53、PTEN在胃癌的发展、浸润、转移中存在相互抑制作用;他们均可以作为胃癌生物学行为和预后判断的独立指标,但三者联合检测准确性可能更高.%Objective To explore the expression and significance of CyclinE,p53 ,and PTEN in gastric carcinoma. Methods Im munohistochemistry Envision two step system was used to detect the expression level of CyclinE, p53 and PTEN in sixty five gas trie cancer samples with thirteen of normal gastric tissue as a control. Results 1. The expression rate of Cyclin E in gastric cancer tissues was high,which went up with increasingly invasive depth,occurring lymphatic metastasis,and clinical stages,reduced histo logical differentiation and long distance metastasis(P-<0. 05). 2. The expression rate of p53 in gastric cancer tissues was high,and increased with increasingly invasive depth and clinical stages,reduced histological differentiation,occurring of lymphatic metastasis and long distance metastasis(P-<0. 05). 3. The expression rate of PTEN in gastric cancer tissues was low,and reduced with in creased invasive depth and clinical stages, reduced histological differentiation,occurring of lymphatic metastasis and long distance metastasis(P-<0. 05). 4. In gastric cancer,the expression of PTEN and p53,Cyclin E were of inverse correlation. The relationship between the expression of Cyclin E and p53 were negative(P<0. 05). Conclusion Cyclin E,p53 and PTEN relatively could be a marker in biological behavior of gastric cancer and estimation of prognosis, then it is suggested that combined detection may have higher accurate rate.