当归多糖对辐射损伤小鼠造血系统保护作用的研究

摘要

Objective To study the effects of angelica polysaccharide(APS) on the damage of bone marrow by ionizing radiation in injured mice,so as to illuminate the protective effects of APS against radioactive haematogenesis injury. Methods Using the line ar accelerator irradiate C57BL/6 mice one time with 4. 0 Gy X ray to establish the animal radiation injured model. Then these mice were killed and extracted BMNC after 7 days injection. Counting the number of the blood cells and BMNCs and observing the pa thology change of bone marrow. The cell cycle and apoptosis rate were detected by flow cytometry and the expression of p53 was detected by immunocytochemistry. Results Compared with normal group,the percentage of Go/Gj phase,apoptosis rate and the ex pression of p53 increased significantly,while the number of the blood cells and BMNCs,the number of cells in bone marrow obvi ously decreased in NS group;both in the 2 mg/kg APS and 8 mg/kg APS group,the percentage of Go/Gi phase and apoptosis rate decreased, while t he number of the blood cells and BMNCs, the number of cells in bone marrow increased. Conclusion APS can improve the fuction of bone marrow hematopoietic system and have protection in ionizing radiation.%目的 研究当归多糖(APS)对电离辐射引起小鼠骨髓损伤的影响,旨在阐明APS对辐射性造血损伤的保护作用.方法 采用直线加速器一次性4.0 Gy剂量全身均匀照射C57BL/6小鼠,建立小鼠放射损伤动物模型.取外周血并进行常规检测,提取骨髓单个核细胞(BMNC)并计数;骨髓切片染色观察骨髓造血细胞数量改变;流式细胞术检测细胞周期,细胞凋亡率;免疫细胞化学法检测p53的表达.结果 与对照组相比,生理盐水(NS)组外周血白细胞(WBC)、红细胞(RBC)、血小板(PLT)及BMNC计数均明显减少,骨髓腔内造血组织显著减少,BMNC G0/G1期比例、凋亡率、p53表达均升高;2 mg/kg APS组和8 mg/kg APS组均能提高外周血WBC、RBC、PLT及BMNC计数,增加骨髓腔内造血细胞数量,降低G0/G1期细胞比例以及细胞凋亡率.结论 APS可以促进骨髓造血系统恢复,对辐射损伤具有良好的保护作用.