目的 探讨维吾尔族妇女宫颈上皮内瘤变(CIN)Ⅰ～Ⅲ级和子宫颈癌组织中miRNA-101的表达情况,并分析子宫颈癌中miRNA-101与人乳头瘤病毒(HPV)感染的关系.方法 采用锁定核苷酸原位杂交技术(LNA-ISH)检测维吾尔族妇女子宫颈鳞状细胞癌、CINⅠ～Ⅲ级和慢性宫颈炎组织中miRNA-101的表达情况,并对子宫颈鳞状细胞癌中miRNA-101与HPV感染情况进行相关性分析.结果 miRNA-101在慢性宫颈炎,CINⅠ、Ⅱ、Ⅲ及子宫颈癌鳞状细胞中的表达率分别为80.00%、73.33%、46.67%、26.67%及10.00%(χ2=36.295,P=0.000).子宫颈鳞状细胞癌中miRNA-101与HPV结果呈负相关(R=-0.767,P=0.000).结论 miRNA-101参与子宫颈鳞状细胞癌的发生、发展,并且与HPV感染有关,miRNA-101可作为子宫颈癌筛查及早期诊断的分子指标之一.%Objective To investigate the expression of miRNA-101 in cervical cancer and cervical intraepithelial neoplasia(CIN) class Ⅰ- Ⅲ in Uighur women and to analyze its relation with human papillomavirus(HPV) infection. Methods The expression of miRNA-101 was detected using the method of locked nucleic acid(LNA)-modified oligonucleotide in situ hybridization(ISH) with the formalin-fixed paraffin-embedded(FFPE) specimens from cervical cancer, chronic cervicitis and CIN class Ⅰ ,Ⅱ , Ⅲ. Then the correlation between the expression of miRNA-101 and HPV infection in cervical cancer was analyzed. Results The expressin rates of miRNA-101 in chronic cervicitis, CIN Ⅰ , Ⅱ , Ⅲ and cervical cancer were 80. 00%, 73. 33%, 46. 67% ,26. 67% and 10. 00 % respectively with significant difference among the five groups. miRNA-101 in cervical cancer was correlated with HPV infection(R = - 0. 767,P = 0. 000). Conclusion miRNA-101 involves in the development and progression of cervical squamous cell cancer and may be related to the HPV infection. miRNA-101 may be used as the molecular marker of screening and early diagnosis in cervical cancer.
展开▼
