重组人肠三叶因子减轻烧伤后肠源性高代谢的实验研究

摘要

目的 探讨重组人肠三叶因子(rhITF)对烧伤后肠源性高代谢的作用及期机制.方法 采用30%体表面积Ⅲ度烧伤小鼠模型,将72只BALB/c小鼠随机分为正常对照组(C组,8只)、烧伤对照组(B组,每个时相点8只)和rhITF治疗组(ITF组,每个时相点8只).观察伤前及烧伤后 1、3、5、7 d小鼠静息能量代谢率(REE)的变化,同时检测伤后血浆内毒素(LPS)、肿瘤坏死因子α(TNFα)、白细胞介素-1(IL-1)和白细胞介素-6(IL-6)的含量.结果 两组烧伤小鼠的REE、TNFα、LPS、IL-1和 IL-6水平均明显高于烧伤前水平(P<0.05),两组比较,ITF组小鼠REE明显低于B组,平均降幅达25%左右.与之对应,血浆LPS、TNF-α、IL-1和 IL-6含量也明显低于B组(P<0.05).结论 rhITF能减轻烧伤后肠道受损程度,降低烧伤小鼠血中炎症介质水平,从而减轻烧伤后肠源性高代谢.%Objective To study the effects of recombinant human intestinal trefoil factor(rhlTF) on enterogenous hyperme-tabolism after severe burn injury and its possible mechanisms. Methods The mouse model of 30% body surface area third degree burns was established. 72 BALB/c mice were randomly divided into three groups: normal control(C,8 cases) , burned control(B,8 cases at each time point) and rhITF treatment(ITF,8 cases at each time point) groups. Resting energy expenditure(REE) ,the level of plasma tumor necrosis factor alpha(TNFa) , lipopolysaccharide(LPS) , interleukin-1 (IL-1) and interleukin-6 (IL-6) were determined before burn and on postburn 1,3,5,7 d. Results After burn injury,the levels of REE,plasma TNFα, LPS,IL-1 and IL-6 in the group B and ITF was significant higher than those before burn(P<0. 05). Compared with the group B,the level of REE in the group ITF were remarkable decreased with the average decreasing amplitude of 25% ,and the plasma TNFa, LPS, IL-1 and IL-6 levels were also lower than those in the group B(P<0. 05). Conclusion rhITF treatment could abate the degree of intestine injury,decrease the inflammatory mediators in blood and reduce the enterogenous hypermitabolism.