APE1和VEGF在非小细胞肺癌组织中的表达及其与肿瘤血管生成和预后的关系

摘要

Objective To study the features of apurinic/apyrimidinic endonuclease 1 (APE1) and vascular endothelial growth factor (VEGF) expression and the correlation with angiogenesis and prognosis in patients with non-small cell lung cancer (NSCLC ) .Methods Expression of APE1 and VEGF was detected by immunohistochemistry in 136 cases with NSCLC .Microves-sel density (MVD) was evaluated by immunohistochemistry with anti-human CD34 antibody .The relationship among APE1 ,VEGF and MVD was analyzed ,and the correlation with disease free survival (DFS) was analyzed in advance .Results Positive rate of APE1 and VEGF was 77 .94% and 66 .18% in NSCLC.The expression of APE1 ,VEGF and MVD showed significant correlations (r=0 .369, P=0.000;r=0.256, P=0 .003 ;r=0 .387, P= 0.000). Tumor size,lymphatic metastasis ,MVD ,APE1 and VEGF expression levels were significantly related with DFS by Kaplan-Meier survival curve(P＜0 .01) .Multivariate analysis by COX regression model showed that tumor size ,lymphatic metastasis and VEGF expression level were the important independent prognosis fac -tor of DFS .Conclusion APE1 may increase angiogenesis by regulating the expression of VEGF .It may be closely related to tumor local recurrence and metastasis ,and APE1 high expression may indicate poor prognosis in NSCLC .%目的 探讨脱嘌呤/脱嘧啶核酸内切酶(APE1)与血管内皮生长因子(VEGF)在非小细胞肺癌(NSCLC)组织中的表达及其与肿瘤血管生成和预后的关系.方法 免疫组化法检测136例NSCLC组织APE1、VEGF的表达,并测定微血管密度(MVD),分析APE1、VEGF 与MVD之间及三者与无病生存时间(DFS)的关系.结果 NSCLC组织中APE1、VEGF高表达率分别为77.94%和66.18%.APE1表达与性别、年龄、病理类型、分化程度、肿瘤大小、淋巴结转移无关,VEGF表达和MVD值与肿瘤大小和淋巴结转移密切相关(P＜0.05).APE1与VEGF、APE1与MVD和VEGF与MVD分别呈正相关(r=0.369,P=0.000;r=0.256,P=0.003;r=0.387,P=0.000).肿瘤大小、淋巴结转移及APE1和VEGF表达、MVD与DFS密切相关(P＜0.05).COX 模型多因素分析显示,肿瘤大小、淋巴结转移和VEGF表达状态是影响NSCLC患者无病生存的独立危险因素.结论 APE1、VEGF的过表达与NSCLC肿瘤血管生成有密切关系,影响NSCLC患者局部复发和远处转移等无病生存预后.