脑钠肽对猪冠状动脉的舒张作用及机制研究

摘要

目的 观察脑钠肽(BNP)对猪冠状动脉舒张作用,探讨其扩血管机制.方法 采用猪冠状动脉环张力测定法,观察脑钠肽对猪冠状动脉的舒张作用及C型利钠肽受体(NPR-C)拮抗剂cANF4-28、鸟苷酸环化酶抑制剂1H-(1,2,4)恶二唑(4,3-α)喹喔啉-1-酮(ODQ)和各种钾离子通道阻滞剂对其舒血管效应的影响.结果 10-6mol/L的BNP对血管的最大舒张率为(68.5±11.5)%,ODQ、四乙胺或格列苯脲均能明显抑制脑肽钠的舒血管作用(P<0.05),cANF4-28对BNP的舒张血管作用无明显影响(P>0.05).结论 BNP可通过激活BKCa和KATP对猪冠状动脉产生舒张作用.%Objective To investigate the vasodilatory effects and mechanisms of brain natriuretic peptide (BNP) in porcine coronary artery. Methods This study was performed on the porcine coronary artery rings. The tension changes of coronary artery ring were recorded with the presence of BNP, further NPR-C antagonist (cΛNF4-28) , guanylate cyclase antagonist (ODQ) and four types of potassium channel blocker were used to explore the vasodilatory mechanisms. Results The maximum vascular relaxation rate of 10 - 6 mol/L BNP was (68. 5 ± 11. 5)%. ODQ,tetraethylammonium or glibenclamide all could inhibit the vascular relaxant activity of BNP (P<0. 05) ,however the action of BNP was not affected by cANF4-28. Conclusion BNP relaxes the coronary arter-y of porcine by activating BKCa and KATP.