大鼠前列腺炎模型中L6～S1背根神经节TRPV1和NGF的表达

摘要

Objective To investigate the express of TRPV1 (transient receptor potential vanilloid-1) and NGF (nerve growth factor)in L6 -S1 dorsal root ganglia(DRG)of chronic prostatitis model in rat. Methods The rat prostatitis model was made by injecting CFA to prostate. The alteration of the express of TRPV1 and NGF in L6 -S1 DRG were observed by Western blot and RT-PCR. Results The express of TRPV1 in L6-S1 DRG of chronic prostatitis model on 7,14 d was significantly higher than that of control group(P<0. 01) , whereas, the express of TRPVl on 7,14 d had no obvious difference. Conclusion Up-regulation of TR-PV1 and NGF in Lg -Si DRG may play an important role in cross organ sensitization following chronic prostatitis.%目的 观察大鼠前列腺炎疼痛模型中L6～S1节段背根神经节(DRG)瞬时感受器电位香草酸受体1(TRPV1)和神经生长因子(NGF)的表达变化.方法 取30只SPF雄性Wistar大鼠随机分为实验组和对照组,分别设0、7、14 d 3个时相,共6组,每组5只,通过前列腺完全弗氏佐剂和生理盐水注射制作大鼠前列腺痛模型和对照组,解剖L6～S1节段DRG组织行蛋白质印迹和逆转录-聚合酶链反应(RT-PCR)检测TRPV1和NGF的表达.结果 慢性前列腺痛大鼠中L6～S1节段DRG的TRPV1和NGF表达在7 d和14 d高于对照组和0 d组,差异有统计学意义(P＜0.01);TRPV1表达在7 d和14 d组间差异无统计学意义(P＞0.05);NGF表达14 d低于7 d(P＜0.05).结论 慢性前列腺炎导致的跨器官痛觉致敏与腰骶段DRG神经元的TRPV1和NGF高表达密切相关.