热休克蛋白-27与磷脂酰肌醇蛋白聚糖-3在原发性肝癌中的表达及意义

摘要

Objective To investigate the clinical significance of heat shock protein 27(HSP-27) and glypican-3(GPC-3) in diag-nosing primary hepatic cancer (PHC) and the clinicopathologic characteristics by detecting their expression in PHC .Methods Im-munohistochemistry was used to detect the expression of HSP-27 and GPC-3 in 32 cases of PHC tissue ,30 cases of live cirrhosis tissue ,25 cases of benign liver occupying tissue and 31 cases of hepatitis tissue .The relationship between their expression and the clinicopathologic features of PHC was analyzed .Results HSP-27 and GPC-3 were highly expressed in the PHC tissues ,the differ-ence had statistical significance compared with their expression levels in the liver cirrhosis tissues ,liver benign neoplasm tissues and hepatitis tissues(P<0 .05) .The expression of HSP-27 in the PHC tissues was correlated with the tumor pathological grade and the TNM stage ,while the expression of GPC-3 was not correlated with the tumor pathological grade and the TNM stage .Conclusion The high expression of HSP-27 and GPC-3 in the PHC tissue is closely related with the tumorigenesis ,progress ,metastasis of PHC ,which may become new pathological diagnosis markers of PHC .%目的：通过检测热休克蛋白-27（HSP-27）、磷脂酰肌醇蛋白聚糖-3（GPC-3）在原发性肝癌（PHC）中的表达，探讨二者在诊断PHC的作用及其临床病理特征的关系。方法：采用免疫组织化学染色法检测32例PHC组织、30例肝硬化组织、25例肝脏良性占位组织及31例肝炎组织中GPC-3、HSP-27的水平，并分析其与PHC的临床因素之间的关系。结果 HSP-27和GPC-3均在肝癌组织中高表达，与肝硬化、肝脏良性占位、肝炎组织中的表达水平比较，差异有统计学意义（P＜0．05）。 HSP-27在肝癌组织的表达与肿瘤的病理分级及TNM分期有关，但GPC-3的表达与肿瘤的病理分级及TNM分期无关。结论： HSP-27、GPC-3在肝癌组织中高表达，与肝癌的发生、发展、转移有关，可能成为新的肿瘤标志物。