IL-12B基因多态性与肝硬化易感性的关联研究

摘要

目的探讨白细胞介素12B（IL-12B）基因多态性与肝硬化易感性的关联。方法收集肝硬化患者173例作为研究组，其生物学父母作为对照组。运用聚合酶链反应扩增及单核苷酸多态性的分子生物学技术，检测 IL-12B基因 rs15677380、rs14050311基因多态性，进行肝硬化与IL-12B基因多态性的关联分析和单体型相对风险率分析。结果 IL-12B基因单核苷酸多态性（SNP）位点rs15677380与肝硬化相关联（P＝0．009），其中等位基因A为危险因素（Z＝2．36），G是保护因素（Z＝-2．36）；rs14050311等位基因多态性与肝硬化相关联（P＝0．013），其中等位基因C是保护因素（Z＝-2．24），T 为危险因素（Z＝2．24）。rs15677380、rs14050311单体型的G/T、A/C与肝硬化有关联（ P＝0．021、0．015，Z＝-1．85、2．16）。结论 IL-12B基因多态性与肝硬化的发生存在关联。%Objective To detect the genetic association between cirrhosis and polymorphism of IL-12B gene .Methods Observed in a sample of 173 parent/offspring trios where the proband net for cirrhosis using correlation analysis and haplotype relative risk a-nalysis .The polymorphism of rs15677380 ,rs14050311 of IL-12B gene was detected with PCR methods and SNP typing in all nucle-us families .Results The rs15677380 allele was connected with cirrhosis(P=0 .009) .Allele G was protect factor(Z= -2 .36) and allele A was the hazard factor(Z=2 .36) .The rs14050311 allele was connected with cirrhosis(P=0 .013) .Allele T was protect fac-tor(Z= -2 .24) and allele C was the hazard factor (Z=2 .24) .The haplotypes of G/T and A/C in the rs15677380 ,rs14050311 were associated with cirrhosis (P=0 .021 ,0 .015 ,Z= -1 .85、2 .16) .Conclusion It shows an association between cirrhosis and the poly-morphism of IL-12B gene in Chinese .