宫颈病变中PI3K-Akt信号通路的表达与HPV感染关系的研究

摘要

目的 探讨磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(Akt)信号通路在宫颈鳞癌(SCC)发生、发展中的意义及与人乳头瘤病毒(HPV)感染的关系.方法 采用免疫组化方法检测PI3K、Akt蛋白在80例SCC,36例宫颈上皮内瘤变(CIN),12例慢性宫颈炎症中的表达；治疗前均采用聚合酶链反应(PCR)方法检测HPV-DNA感染.结果 SCC组中PI3K、Akt蛋白阳性表达率显著高于CIN组和慢性宫颈炎症组(P＜0.05),SCC中PI3K、Akt蛋白的阳性表达均与临床分期密切相关(P＜0.05)；PI3K、Akt在SCC中的表达存在相关关系(P＜0 05)；116例CIN和SCC中,PI3K-Akt在HPV阳性组的表达率均显著高于HPV阴性组(P＜0 05).结论 PI3K、Akt在SCC发生、发展中起重要作用；两者与HPV感染密切相关.%Objective To explore the expression and clinical significance of PI3K-Akt in cervical lesion and the relationship with HPV.Methods Immunohistochemical method was used to test the expression of PI3K and Akt protein in 80 cases of SCC,36 cases of CIN and 12 cases of cervicitis; HPV-DNA was detected by polymerase chain reaction technique.Results In SCC,the expression of PI3K and Akt were significantly higher than these of CIN and cervicitis(P＜0.05) ;the expression of PI3K and Akt was significantly correlated with the clinical stage(P＜0.05) ;the expression of PI3K was correlated with the expression of Akt(P＜0.05) ;in 116 cases of SCC and CIN,when there were H PV infection,the expression of PI3K and Akt were significantly higher than no infection.Conclusion PI3K and Akt should play an important role in the genesis and development of SCC,both have a close relationship with HPV infection.