Objective To observe tumor metastasis suppressor genes KISS-1 expression in stomach cancer tissue and it's function to extracellular matrix. Methods Collect 38 cases of patient's stomach organizations with stomach cancer and 38 cases of normal gastric mucosa tissue from January 2010 to February 2012 in our hospital, use RT-PCR tested the specimens KISS-1 expression in two groups, and testing various KISS-1 under positive level, the extracellular matrix (ECM ) differences in the level of expression. Results The positive rate of KISS-1 mRNA in stomach tissue is 56. 2%, in normal gastric mucosa tissue of the positive rate was 87.1%, the differences between them are statistically significant (P＜0.01).And according to the different expression level of KISS-1, ECM's main ingredients :laminin(LN ), Ⅳ protein collagen type and fibronectin (FN ) expressing a statistically significant difference( P＜0. 05 ). Conclusion KISS-1 and the occurrence and metastasis of cancer of the stomach is closely related, and through to the influence of the ECM expressed indirectly affects tumors and development process.%目的 观察胃癌组织中肿瘤转移抑制基因KISS-1的表达水平与胃癌浸润、转移的关系及其对细胞外基质的作用.方法 收集2011年10月至2012年6月在通辽市科尔沁区第一人民医院治疗的38例胃癌患者的胃癌组织与38例正常胃黏膜组织,采用逆转录-聚合酶链反应(RT-PCR)检测两组标本中KISS-1表达情况,同时检测不同KISS-1阳性水平下细胞外基质(ECM)的表达水平差异.结果 KISS-1 信使RNA(mRNA)在胃癌组织中的阳性率为56.2%,在正常胃黏膜组织中的阳性率为87.1%,二者比较差异有统计学意义(P＜0.01).且不同KISS-1阳性水平下,ECM的主要成分:层粘连蛋白(LN)、Ⅳ型胶原及纤维连接蛋白(FN)表达水平比较差异有统计学意义(P＜0.05).结论 胃癌组织中肿瘤转移抑制基因KISS-1的表达水平与胃癌浸润、转移密切相关,并通过对ECM表达的影响间接作用于肿瘤的发生与发展过程.
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