丝裂霉素纤维蛋白胶凝胶瘤内注射治疗裸鼠肝癌

摘要

Objective To investigate the therapeutic effects of intratumoral injection of mitomycin C mixing with fibrin glue (MMC-FG) on nude mice with liver cancer and provide experimental foundation for its clinical application.Methods 30 nude mice with subcutaneous human hepatoma were randomized into five groups,in which MMC-FG was injected intratumorally(group A),MMC-FG injected intraperitoneally(group B),mitomycin C(MMC) injected intratumorally(group C),normal FG injected intratumorally(group D) and normal saline injected intratumorally(group E) respectively.After administation individual tumor growth was observed,tumor weight was scaled,inhibition ratio of tumor weight was calculated,and white blood count(WBC) and platelet(PLT)of peripheral blood of nude mice were examined.Other 50 nude mice with liver cancer were randomized into five groups in the same way.Survival time of individual nude mice was observed after administation likewise.Results The tumor volume of nude mice in group A increased the most slowly,whose inhibition ratio of tumor weight was 53.40％ and tumor weight was much lighter than that in other groups(P＜0.05).WBC and PLT of nude mice in group C were lower than those in group A(P＜0.05),while were similar with those in group B,D or group E(P＞0.05).Survival time of nude mice in group A was longer than that in other groups (P＜0.05).Conclusion Intratumoral injection of MMC-FG can inhibit tumor growth effectively,decrease toxicity of MMC,and prolong survival time of nude mice.It is an effective,safe and simple chemotherapeutic method.%目的 探讨丝裂霉素纤维蛋白胶凝胶(MMC-FG)瘤内注射对裸鼠肝癌的治疗效果,为其临床应用提供实验依据.方法 30只成功建模的皮下荷肝癌裸鼠随机分成5组,分别是瘤内注射MMC-FG组(A组)、腹腔内注射MMC-FG组(B组)、瘤内注射丝裂霉素MMC组(C组)、瘤内注射FG组(D组)、瘤内注射生理盐水组(E组).给药后观察裸鼠肿瘤生长情况,称取肿瘤重量并计算瘤重抑制率,检测裸鼠外周血液白细胞(WBC)及血小板(PLT).另50只荷肝癌裸鼠随机分成上述5组,同法给药后观察各组裸鼠的生存时间.结果 A组裸鼠肿瘤体积增长最缓慢,瘤重抑制率为53.40％,肿瘤重量明显低于其他组(P＜0.05).C组裸鼠外周血液WBC及PLT检测低于A组(P＜0.05),与B、D组及E组均无明显差异(P＞0.05).A组裸鼠的生存时间长于其他组(P＜0.05).结论 瘤内注射MMC-FG可有效抑制肿瘤生长,减小MMC的不良反应,延长裸鼠生存时间,是一种有效、安全、简便的化疗方法.