肺结核合并糖尿病患者血清IL-2、TNF-α的表达及临床意义

摘要

目的：观察肺结核合并糖尿病患者血清 IL-2、TNF-α的表达，并初步探讨其临床意义。方法试验分为对照组（门诊健康体检者，n＝10）、肺结核组（n＝20）、糖尿病组（n＝20）和肺结核合并糖尿病组（n＝20），治疗2个月后根据病情转归将肺结核合并糖尿病组分为好转组及恶化组，ELISA法检测血清中 IL-2、TNF-α水平。结果治疗前：肺结核组、糖尿病组、肺结核合并糖尿病组血清 IL-2水平均降低（P＜0．05），其中肺结核合并糖尿病组低于肺结核组和糖尿病组（P＜0．05）；肺结核合并糖尿病组、肺结核组、糖尿病组血清TNF-α水平均升高（P＜0．05），其中肺结核合并糖尿病组高于糖尿病组（P＜0．05）；治疗后：肺结核合并糖尿病好转组血清 IL-2水平上升，而TNF-α水平有明显下降（P＜0．05），恶化组治疗前后变化不明显（P＞0．05）。结论IL-2、TNF-α在肺结核合并糖尿病的免疫发病机制中起着一定的作用，其动态变化能反应肺结核合并糖尿病的病情发展及转归。%Objective To investigate the expression of serum interleukin-2(IL-2),tumor necrosis factor-α(TNF-α)in the pa-tients with pulmonary tuberculosis and diabetes,and prelininary explore its clinical significance.Methods Experiment were divided into control group(health check-up outpatient,n=10),pulmonary tuberculosis group(n=20),diabetes group(n=20),tuberculosis combine diabetes group(n=20),tuberculosis combine diabetes group was divided into improved group,diabetes group and deterio-rated group after 2 months with therapy according to disease outcome,ELISA assay was used to measured the level of serum IL-2 and TNF-α.Results Before treatment:serum IL-2 levels of pulmonary tuberculosis group,diabetes group and pulmonary tuberculo-sis combine diabetes group were reduced (P<0.05),and the pulmonary tuberculosis group was lower than the pulmonary tubercu-losis group and diabetes group (P<0.05);serum TNF-αlevels of pulmonary tuberculosis combine diabetes group,pulmonary tu-berculosis group and diabetes group increased (P<0.05),and that of pulmonary tuberculosis combine diabetes group was higher than that of diabetes group (P<0.05).After treatment:the level of serum IL-2 increased and TNF-αsignificantly decreased in pul-monary tuberculosis combine diabetes improved group (P<0.05),the deterioration group had no change(P>0.05).Conclusion IL-2 and TNF-αplay a role in the immune pathogenesis of tuberculosis combine diabetes,which dynamic changes can reflect the progression and outcome of pulmonary tuberculosis combine diabetes.