降钙素对骨质疏松性骨折骨痂血管内皮生长因子表达的作用

摘要

目的：观察降钙素对去卵巢大鼠股骨骨折愈合过程中骨痂组织血管内皮生长因子表达的作用。方法选择3月龄雌性SD大鼠80只，随机分为假手术组（n＝10）、卵巢切除组（n＝10）、对照组（n＝30）和用药组（n＝30）。卵巢切除后4周处死假手术组和卵巢切除组大鼠，测量右股骨骨密度。卵巢切除4周后，对照组和用药组行右股骨中段骨折，分别皮下注射生理盐水和降钙素（16 IU/kg），隔日1次，于骨折后3、6、9周处死大鼠取右股骨测骨密度，行苏木精-伊红染色（HE染色），血管内皮生长因子免疫组化染色。结果与假手术组比较，卵巢切除组大鼠体重显著增加（P＜0．05），股骨骨密度显著降低（P＜0．05）。 HE染色：骨折后3周，两组骨折愈合以软骨内成骨过程为主，骨密度无显著性差异（P＞0．05）；骨折后6周，两组骨小梁排列较有序，用药组股骨骨密度较对照组升高（P＜0．05）；骨折后9周，两组均以骨性骨痂为主，骨小梁排列紧密，骨密度在两组间具有显著性差异（P＜0．05）。对照组与用药组在骨折后3、6、9周骨痂组织的血管内皮生长因子（VEGF）免疫组化染色结果无显著性差异（P＞0．05）。结论降钙素治疗能增加骨质疏松性骨折骨密度，对骨痂组织VEGF的表达无明显影响。%Objective To study the effect of calcitonin on the expression of vascular endothelial growth factor in femoral fracture healing in ovariectomized rats .Methods 80 female rats of 3 months old were randomly divided into four groups :Sham operation group(Sham ,10 cases);Ovariectomized operation group(OVX ,10 cases);Ovariectomy+ fracture+ salinegroup(Control ,30 cases) and Ovariectomy+ fracture +calcitonin group(Experimental ,30 cases) .Rats in Sham group and OVX group were performed ovari-otomy and killed after 4 weeks ,femoral bone mineral density was measured .For rats in Control group and Experimental group ,right middle femoral facture was performed 4 weeks later after ovariectomy .Calcitonin(16 IU/kg)were injected subcutaneously once per 2 days in Experimental group ,while those in Control group were given equal volume of normal saline .Rods in these two groups were killed after 3 ,6 ,9 weeks(10 at a time) ,femoral bone mineral density(BMD)was measured;hematoxylin-eosin staining and im-munohisto-chemical staining were performed at the 3th ,6th and 9th week after fracture ,respectively .Results Compared with Sham group ,rats weight in OVX group gained more(P< 0 .05) ,and BMD in OVX group significantly decreased more(P< 0 .05) .HE staining ,at the 3th week after fracture ,endochondral ossification domained in fracture healing ,and no significant difference existed (P>0 .05)in BMD .At the 6th week after fracture ,bone trabecula in both groups arranged in order ,compared with the control group ,BMD in experimental group increased significantly(P<0 .05) .At the 9th week after fracture ,bone trabecula in both groups arranged closely ,BMD in both groups showed significant difference(P<0 .05) .There was no significant difference of vascular endo-thelial growth factor(VEGF)between experimental group and control group at the 3th ,6th ,9th week after fracture(P>0 .05) .Con-clusion The results suggest that calcitonin treatment could enhance the bone mineral density significantly after fracture ,but it has no impact on the expression of VEGF in osteoporotic fracture healing .