Survivin蛋白和 PT EN 基因在直肠癌中的表达及其意义

摘要

Objective To detect the expression of Survivin ,PTEN and to discuss the correlations between them and the clinico-pathological characteristic of rectal carcinoma ,evaluate their effects on the development of rectal carcinoma .Methods SP immuno-histochemical was used to detect the expression level of Survivin and PTEN in 72 rectal carcinoma samples ,and randomly selected 20 cases of normal paraneoplastic tissues as control group .Results The positive rate of Survivin and PTEN in normal paraneoplas-tic tissues were 0 and 85 .0% respectively ;The positive rate in rectal carcinoma samples were 61 .1% and 41 .7% respectively .Posi-tive expressions of Survivin and PTEN did not had no correlation with gender ,age ,but correlated with differentiation ,dukes staging and lymph node metastasis .PTEN expression was negatively correlated with Survivin expression in rectal carcinoma .Conclusion The low expression of Survivin and high expression of PTEN might promote tumor genesis and progression of rectal carcinoma , which could be used to judge the rectal cancer clinical staging and pathologic grading .%目的：观察Survivin蛋白和PT EN基因在直肠癌中的表达，分析二者与直肠癌临床病理特征的关系，评价其对直肠癌发生发展的影响。方法采用链霉菌抗生物素蛋白-过氧化物酶连结法，检测Survivin和PT EN在72例直肠癌组织中的表达，并随机抽取20例癌旁正常组织作为对照组。结果在癌旁正常组织中，Survivin的阳性表达率为0，PT EN 的阳性表达率为85．0％；在直肠癌组织中，Survivin的阳性表达率为61．1％，PT EN的阳性表达率为41．7％。Survivin和PT EN的表达与直肠癌患者的性别、年龄无关，与分化程度、Dukes分期和淋巴结转移有关。Survivin与 PT EN在直肠癌中的表达呈负相关。结论直肠癌发生发展的相关因素中，PT EN的低表达和Survivin的过表达可能起重要作用，可作为判断直肠癌分期分级的指标。