乳腺癌术后骨转移患者血清差异蛋白的质谱分析

摘要

目的：获取乳腺癌术后骨转移患者和无转移患者的血清肽质量指纹图谱（PM F ），筛选并鉴定与乳腺癌骨转移相关的血清差异蛋白，同时建立乳腺癌骨转移的诊断模型。方法采用Clinprot液体芯片飞行时间质谱系统（ClinprotTM M ALDI-TOF MS）获得两组血清标本（单纯骨转移及无转移患者各18例）的PMF ，然后使用内嵌的统计分析软件筛选两组血清标本中有统计意义的差异蛋白峰。每组样品检测2次。结果所有标本5d后重复检测，2次质谱图基本一致，随机选择4个蛋白峰计算变异系数（CV ）均小于30％，说明该系统具有良好的可重复性。两组患者血清的PM F相比较，蛋白峰差异无统计学意义（ P＞0．05）。结论乳腺癌术后单纯骨转移与无转移患者无血清差异蛋白存在，不能通过检测外周血的方法筛选乳腺癌骨转移差异蛋白。%Objective To obtain the peptide mass fingerprintings(PMF)of serums of the patients with bone metastasis and with-out metastasis and to filtrate and identify the differential serum proteins of patients with bone metastasis after radical mastectomy . To establish diagnostic models for diagnosis of bone metastasis after breast cancer operation .Methods Two groups of serum sam-ples were analyzed by ClinprotTM MALDI-TOF MS and gain PMF ,18 samples from patients with merely bone metastasis and 18 samples from patients without metastasis .Characteristic protein peaks were analyzed and selected by analyses software within Clin-prot system .Every group was repeated 2 times .Results All serum samples were repeated after 5 days and the fingerprintings were similar to the former .4 protein peaks were selected randomly to compute coefficient of variation which are less than 30% .The Clin-prot system has excellent repeatability .No differential protein was detected by analyzing PMF (P>0 .05) .Conclusion No differen-tial serum protein exists in patients with bone metastasis and without metastasis and detecting differential protein in peripheral blood may be impossible .