乳腺癌17号染色体异倍体对HER-2表达的影响及临床病理学意义

摘要

目的：探讨乳腺癌17染色体异倍体（多体、单体）对人表皮生长因子受体-2（HER-2）表达的影响及其临床病理学意义。方法收集2013年1～12月，同时采用免疫组化（IHC）和荧光原位杂交（FISH）技术检测 HER-2表达状态的乳腺浸润癌病例，共352例。根据17号染色体多体、单体及HER-2的表达情况分组，对各组患者的临床病理特征进行比较分析。结果17号染色体多体、单体与 H ER-2蛋白表达无相关性（ P＞0.05）；17号染色体多体与 H ER-2基因扩增呈负相关（ r＝—0.162，P＝0.001）；17号染色体单体与 HER-2基因扩增呈正相关（r＝0.282，P＝0.000）。17号染色体多体组与HER-2阴性组比较，组织学分级，脉管瘤栓差异有统计学意义（P＜0.05）。17号染色体多体组与 HER-2阳性组比较，组织学分级，脉管瘤栓，Ki-67表达， ER、PR阳性表达差异有统计学意义（P＜0.05）。17号染色体单体组与HER-2阴性组比较，PR阳性表达差异有统计学意义（P＜0.05）。17号染色体单体组与 HER-2阳性组比较，肿瘤大小差异有统计学意义（P＜0.05）。17号染色体多体组与17号染色体单体组比较，脉管瘤栓，PR阳性表达差异有统计学意义（P＜0.05）。结论17号染色体多体、单体与HER-2的蛋白表达无相关性，而与H ER-2基因扩增有相关性。17号染色体多体组具有独特的临床病理特点，可能提示乳腺癌的预后。%Objective To investigate the impact of chromosome 17 heteroploid (polysomy ,haploid) of breast cancer on the expression of human epidermal growth factor receptor 2(HER‐2) and its clinicopathologic significance .Methods A total of 352 cases of invasive breast cancer were collected from January to December 2013 .The expression of HER‐2 was detected by simultane‐ously using the fluorescent insituhybridization(FISH) and mimunohistochemistry .The cases were divided into the groups according to the chromosome 17 polysomy ,haploid and the expression of HER‐2 .The clinicopathological features were analyzed and compared among groups .Results The chromosome 17 polysomy and haploid had no correlation with HER‐2 protein expression(P>0 .05);chromosome 17 polysomy was negatively correlated with HER‐2 gene amplification(r= -0 .162 ,P=0 .001);chromosome 17 hap‐loid was positively correlated with HER‐2 gene amplification(r=0 .282 ,P=0 .000) .Compared with the HER‐2‐negative group ,the chromosome 17 polysomy group had a statistically significant difference in histological grade and vascular tumor thrombus (P<0 .05) .Compared with the HER‐2‐positive group ,the chromosome 17 polysomy group had a statistically significant difference in histological grade ,vascular tumor thrombus ,Ki‐67 expression ,ER positive expression and PR positive expression (P<0 .05) .Com‐pared with the HER‐2‐negative group ,the chromosome 17 haploid group had a statistically significant difference in PR positive ex‐pression (P<0 .05) .The tumor diameter in the chromosome 17 haploid group had a statistically significant difference compared with the HER‐2‐positive group(P<0 .05) .Compared with the chromosome 17 haploid group ,the chromosome 17 polysomy group had a statistically significant difference in vascular tumor thrombus and PR positive expression(P<0 .05) .Conclusion The chro‐mosome 17 polysomy and haploid have no correlation with the HER‐2 protein expression ,but associated with HER‐2 gene amplifi‐cation .The chromosome 17 polysomy group has unique clinicopathological features ,which may indicate the prognosis in the patients with breast cancer .