3种方法治疗肠道菌群失调合并过敏性哮喘大鼠对肠道菌群的影响

摘要

目的 根据“肺与大肠相表里”的理论,为肺肠合治法治疗肠道菌群失调并过敏性哮喘提供理论依据.方法 实验共分了空白组、模型组、阳性组和3种中药复方的给药组;采用头孢哌酮加白色念珠菌灌胃SD大鼠的方式复制肠道菌群失调,并在此基础上运用卵清清蛋白注射致敏与雾化诱发哮喘,雾化同时分别给予加味升降散、桂枝加厚朴杏子汤和增液承气汤药液从肺肠合治、治肺和治肠进行治疗;采用Buxco全身体积描记系统分析呼吸功能,通过16SrDNA分析肠道菌群的变化.结果 根据OTU值分析各组肠道菌群物种组成发现,各组对乳酸杆菌的恢复接近空白组且优于阳性组,3个治疗组均可促进变形菌门和拟杆菌门菌群的恢复,但肺肠合治效果最佳,通过Alpha多样性分析发现肠道微生物菌群多样性从高到低分别是治肠组、治肺组、肺肠合治组、模型组;呼吸频率和气道高反应性模型组为260.56(1.28),治疗后肺肠合治组、治肺组和治肠组分别为141.79(0.93)、137.25(1.30)和203.16(0.84),各治疗组大鼠呼吸频率均低于模型组,而气道高反应性只有肺肠合治组和治肠组低于模型组且差异有统计学意义(P＜0.05).结论 肺肠合治法治疗肠道菌群失调合并过敏性哮喘大鼠在肺功能和肠道菌群恢复上优于单纯治肠或治肺法.%Objective To provide a theoretical basis for the pulmonary enteric therapy in treating intestinal dysbacteriosis complicating allergic asthma according to the traditional theory of "Lung and Large intestine are interiorly-exteriorly".Methods The experiment included the blank group,model group,positive control group and three kinds of traditional Chinese medicine compound administration group;the intestinal dysbacteriosis in SD rats was duplicated by adopting cefoperazone and candida albicans gavage.On this basis ovalbumin injection sensitization and nebulization were applied to induce asthma.Meanwhile Jia-Wei-ShengJiang Powder(SJS),Guizhi-Jia-Houpu-Xingzi Decoction(GHX) and Zeng-Ye-Cheng-Qi Decoction(ZYCQ) were orally administered for treatment of lung-intestine combine therapy,lung therapy and intestine therapy.The respiratory function was analyzed by using the Buxco whole body plethysmography.The variety of intestinal flora were analyzed by using 16SrDNA.Results Analyzing the specieces composition of intestinal flora in each group by the OUT value found that the recovery of Lactobacillus in each group was close to that in the blank group and better than that in the positive group,meanwhile,3 treatment groups all could promote the recovery of Proteobacteria and bacteroidetes,but the lung-intestine combine therapy had optimal effect,the Alpha diversity analysis found that the intestinal microflora diversities from high to low were in turn the intestinal therapy group,lung therapy group,lungintestine combined therapy group and model group;the respiratory frequency and airway hyperreactivity in the model group was 260.56(1.28),which in the lung-intestine combined therapy group,lung therapy and intestine therapy after treatment were 141.79 (0.93),137.25 (1.30) and 203.16 (0.84) respectively,the rat respiratory frequency in each treatment group was lower than that in the model group,while the airway hyperreactivity only in the lung-intestine combined therapy group and intestinal therapy group was lower than that in the model group,moreover showing statistical significance.Conclusion The lung-intestine combine therapy method for treating rat intestinal dysbacteriosis complicating allergic asthma is superior to simple lung therapy method or intestine therapy method.