东北汉族群体19个Y-STR基因座多态性和东北亚地区各民族的聚类分析

摘要

目的 研究东北汉族群体的19个Y-STR基因座的遗传多态性和东北亚15个群体间的遗传关系,探讨其法医学和群体遗传学研究的应用价值.方法 采用Y-filer复合扩增体系对200例东北汉族无血缘关系的男性个体的19个Y-STR基因座进行扩增,应用ABI310分析仪进行基因分型,采用Arelquin3.11、Mega4.1和SPSS17.0软件进行群体间的AMOVA分析、聚类分析和多维标度分析.结果 东北汉族群体的19个Y-STR基因座的基因多态性普遍较高,共检出200个单倍型,单倍型多态性在0.350 9(DYS391)～0.971 1(DYS385a/b),适合应用于法医学个体识别和亲权鉴定;东北亚15个群体间存在差异,遗传距离在0.000 9～0.643 2,群体间聚类分析和多维度分析结果与民族起源和迁徙史基本相一致.结论 东北汉族群体的19个Y-STR基因座的基因多态性普遍较高,适合应用于法医学个体识别和亲权鉴定中.%Objective To investigate the polymorphisms of the 19 Y-STR loci in the Han population in Northeast China,and analyze the genetic relationships of 15 ethnic groups in northeast Asia region,and to evaluate their forensic value and population genetic value.Methods The 19 Y-STR unrelated Han males in 200 unrelated Han males in Northeast China were amplified with the Y-filer system,and the PCR products were analyzed by the 310 Genetic Analyzer.The AMOVA analysis,cluster analysis and MDS analysis were calculated by Arelquin3.11,Mega4.1 and SPSS17.0.Results The polymorphisms of 19 Y-STR loci in the Han population in Northeast China had generally higher gene diversity value which were ranged from 0.350 9 (DYS391) to 0.971 1 (DYS385a/b),and totally 200 haplotypes were observed.The 19 Y-STR loci displayed high genetic polymorphisms in the Han population in Northeast China,indicating that these 19 loci were useful genetic markers for forensic personal identification and paternity testing.There were distinctions among 15 ethnic groups.The genetic distance between 15 ethnic groups were ranged from 0.000 9 to 0.643 2,and the conclusion of cluster analysis and MDS analysis were similar to the ethnogeny research and ethnic migration history.Conclusion The 19 Y-STR loci displayed high genetic polymorphisms in the Han population in Northeast China,and these 19 loci were useful genetic markers for forensic personal identification and paternity testing.