为了开发一种低毒高效的新型抗烟草花叶病毒抑制剂，利用合成的新型寡糖希夫碱衍生物进行了抗烟草花叶病毒的药效研究。结果表明：（1）在珊西烟上，采用枯斑寄主半叶法进行抗烟草花叶病毒的药剂筛选，壳寡糖及其衍生物都能有效减少叶片感染烟草花叶病毒的枯斑数，以枯斑抑制率为66.39%的水杨醛席夫碱的预防效果最好；（2）在普通烟K326上，壳寡糖及其衍生物可降低侵染病毒烟草中的叶绿素下降幅度，还可以提高叶片中的防御酶超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和苯丙氨酸解氨酶（PAL）的活性，脯氨酸的含量也得到了积累。%In order to develop a kind of the new inhibitor of low toxicity and high efficiency against TMV, the inhibitive effect of the new oligosaccharide Schiff base derivatives against TMV was conducted. The results showed that: (1) on Xanthi-NN, chitosan and its derivatives could all reduce the number of the dry spots infected by TMV effectively by screening potions with the dry spot host half leaf method, and the best result was obtained for salicylaldehyde chitosan Schiff base giving the inhibition rate of 66.39%; (2) on Nicotiana tabacum K326, chitosan and its derivatives can lower the decrease of chlorophyll content, and increase activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) in inoculated leaves, at the same time the content of proline had also accumulated.
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