PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aque- ous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10―35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.
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机译:6-O-(Hydroxypropyltrimethylammonia)-beta-cyclodextrin with Low Degree of Substitution: Convenient Preparation and its Application as a Chiral Selector in Capillary Electrophoresis
机译:How the Group Leaders of Lesson Preparation Groups Facilitate Group Learning and Reflective Practice in Chinese Public High Schools =中国公立高中的备课组长是如何引领团队学习和反思性实践的?
机译:Erratum for “Preparation of Low-Temperature Phase Change Materials Microcapsules and Its Application to Asphalt Pavement” by Feng Li, Siqi Zhou, Sai Chen, Zhenglong Yang, Jian Yang, Xingyi Zhu, Yuchuan Du, Peiting Zhou, and Zhihao Cheng