绝经后女性盆底功能障碍患者肛提肌中抗肌营养不良蛋白和结蛋白表达及意义

摘要

Objective To investigate the association of mRNA expressions of desmin and dystrophin in the levator ani muscle with pelvic floor dysfunction (PFD) in postmenopausal women.Methods The present study enrolled 30 patients with stress urinary incontinence (SUI) (SUI group),30 patients with pelvic organ prolapse (POP) (POP group) and 6 controls with rectal cancer but free from SUI or POP (control group).Biopsy of the levator ani muscle was conducted for all the subjects during surgery.Real-time PCR was used to determine quantitatively the mRNA levels of desmin and dystrophin in the levator ani muscle.Results The mRNA expressions of desmin and dystrophin were significantly decreased in the SUI and POP groups when compared with the control group (P＜0.01).There was no significant difference regarding the mRNA expressions of desmin and dystrophin between the SUI and POP groups.Conclusion The significantly reduced mRNA expressions of desmin and dystrophin may contribute to the pathogenesis of PFD in postmenopausal women.%目的 探讨抗肌营养不良蛋白和结蛋白mRNA的表达与绝经后女性盆底功能障碍(PFD)的关系.方法 选择30例压力性尿失禁患者(SUI组)、30例盆底组织膨出患者(POP组)及6例无SUI和POP的直肠癌患者(对照组),术中行肛提肌活组织检查(活检),应用实时荧光定量聚合酶链反应(PCR)的方法检测肛提肌中抗肌营养不良蛋白和结蛋白的表达.结果 POP组和SUI组肛提肌中肌营养不良蛋白和结蛋白mRNA表达较对照组均降低(P＜0.01).POP组和SUI组比较,肛提肌中抗肌营养不良蛋白和结蛋白mRNA表达差异无统计学意义(P＞0.05).结论 绝经后女性盆底功能障碍性疾病的发生与女性肛提肌中抗肌营养不良蛋白及结蛋白的表达降低有关.