Objective To study the expression of multi-drug resistance protein P-glycoprotein (P-gp) and lym-phocyte subset count in patients with systemic lupus erythematosus (SLE), and to explore the possible mechanisms in predicting drug resistance. Methods Twenty patients with treatment-naive SLE and 15 healthy controls were en-rolled. The expression of peripheral blood lymphocytes P-gp and the lymphocyte subset count were detected by flow cytometry. This entailed analysis on the association of P-glycoprotein and lymphocyte subsets, including CD4+T cell or helper T cells (Th), CD8+T cells, natural killer cells (NK), total T lymphocytes and B lymphocytes in patients with SLE. Statistical analyses, including t-test for independent samples, χ2 test for quantitative data and Pearson′s correla-tion analysis, were conducted by using SPSS 17.0. Results Mean relative fluorescence intensity (RFI) of P-gp was significantly higher in patients with SLE [(3.3±2.0) vs (2.2±0.7), t=18.8, P0.05), but P-gp expression was significantly correlated with CD4+T cells and the ratio of CD4+/CD8+cells (r=0.602, P=0.002; r=0.561, P=0.005). In patients with increased CD4+T cells and the ratio of CD4+/CD8+cells, the P-gp was positively correlated with RFI (r=0.713, P=0.025; r=0.628, P=0.014). There was no correlation between the expression of P-gp and sex and the age of onset in patients with SLE. Conclusion The expression of peripheral blood lymphocytes P-gp is an index reflecting multi-drug resistance in SLE. The expression of P-gp is significant correlated with the number of CD4+T cells and the ratio of CD4+/CD8+cells in the abnormal lymphocyte subsets, suggesting that P-gp may be an indicator of the efficacy of drug treatment, and may play an important role to improve prognosis and reduce the drug resistance in SLE.%目的：探讨多药耐药蛋白P-糖蛋白（P-gp）的表达水平与淋巴细胞亚群计数在系统性红斑狼疮（SLE）患者中的关系及在预测疾病耐药性方面可能的机制。方法选择初发SLE患者20例和健康对照组15名。通过流式细胞术测定外周血淋巴细胞P-gp的表达水平和淋巴细胞亚群的计数。分析SLE患者外周血淋巴细胞P-gp的表达水平与淋巴细胞亚群中CD4＋T细胞，CD8＋T细胞、自然杀伤细胞、总T细胞和总B细胞等的相关性。采用t检验和χ2检验、Pearson相关性分析进行统计学分析。结果①健康对照组与SLE患者外周血淋巴细胞的相对荧光强度（RFI）平均值分别为2．2±0．7、3．3±2．0，差异有统计学意义（t＝18．8，P＜0．01）。②SLE患者外周血淋巴细胞P-gp的表达与淋巴细胞亚群中总T细胞，总B细胞，NK细胞的计数均无相关性，但P-gp的表达水平与CD4＋T细胞、CD4＋/CD8＋比值有相关性（r＝0．602，P＝0．002；r＝0．561，P＝0．005）。③根据SLE患者外周血淋巴细胞亚群计数分为增高组和降低组，分别与相应的RFI值比较，发现CD4＋T细胞及CD4＋/CD8＋比值的增高组与相应P-gp的表达呈正相关（r＝0．713，P＝0．025；r＝0．628，P＝0．014）。结论外周血淋巴细胞的P-gp表达是SLE患者多药耐药性的指标，SLE患者药物跨膜转运蛋白P-gp表达水平尚与其存在免疫功能异常亢进淋巴细胞亚群中CD4＋T细胞及CD4＋/CD8＋比值密切联系，提示P-gp可能是药物治疗疗效的指标，对改善预后和联合治疗减少耐药有重要的意义。
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