地塞米松对肺纤维化大鼠肺单核细胞rn趋化蛋白-1基因表达的影响

摘要

目的研究地塞米松（Dxs）对肺纤维化大鼠肺单核细胞趋化蛋白-1（MCP-1）mRNA表达的影响，阐明糖皮质激素治疗肺纤维化疾病的分子机制。方法气管内滴注博莱霉素致大鼠肺纤维化模型，不同浓度的地塞米松处理后，观察肺组织的病理学变化和计数5×5 mm高倍视野内炎症细胞数目;RT-PCR方法检测肺组织MCP-1 mRNA表达水平。结果地塞米松减少了肺组织炎症细胞的聚集，延缓了肺纤维化的形成过程，并抑制了MCP-1 mRNA的表达，呈现一定的剂量依赖效应趋势。结论地塞米松治疗肺纤维化疾病的分子机制与抑制肺MCP-1基因表达有关。%AIM　To study the effects of dexamethasone on expressing monocyte chemoattractant protein-1(MCP-1 ) mRNA in the rats with pulmonary fibrosis, elaborate the molecular mechanism of dexamethasone (Dxs) in pulmonary fibrosis therapy. METHODS　The model of pulmonary fibrosis was established by instilling bleomycin intratracheally. After treating with Dxsip, the levels of MCP-1 mRNA were determined by RT-PCR. The histological changes were observed and the numbers of inflammatory cells were counted in optical microscopy field. RESULTS　The accumulation of inflammatory cells decreased markedly, and the symptom of pulmonary fibrosis was alleviated. Furthermore, Dxs evidently inhibited the expression of MCP-1 mRNA in lung tissues with pulmonary fibrosis. CONCLUSION　The molecular mechanism of Dxs in pulmonary fibrosis therapy was associated with inhibiting the expression of MCP-1 mRNA.