目的 观察原儿茶酸调节体外培养的神经干/祖细胞分化及对细胞分化显型的保护作用.方法 神经干/祖细胞取自胚胎14 d小鼠海马组织,以单层贴壁方式培养.免疫荧光染色标记分化的细胞,CCK-8试剂盒检测细胞的生存力,流式细胞仪分析凋亡细胞.结果 原儿茶酸单独使用不能诱导神经干/祖细胞的分化,但与1%胎牛血清的联合应用时,原儿茶酸(0.06 mmol·L-1)有效地提高神经元的分化比例,介导神经元的成熟并促进突触的伸长;另一方面,原儿茶酸明显提高了分化显型的生存力并抑制了细胞凋亡,同时激活了细胞内源性的抗氧化酶系统.结论 原儿茶酸能够有效地促进神经干/祖细胞分化为神经元的比例并提高细胞分化显型的生存力.%Aim To investigate the protective effects of protocatechuic acid ( PCA ) on cultured neural stem/ progenitor cells ( NS/PCs ) differentiation. Methods NS/PCs obtained from 14-day-old rat embryos were cultured under differentiation condition in the monolay-er manner with or without PCA for the indicated time. Phenotypes differentiated from NS/PCs were visualized by immunofluorescent staining, the cell viability was determined by Cell Counting Kit-8 ( CCK-8 ) test, and cell apoptosis was assayed by flow cytometric analysis. Results PCA could promote the neuronal differentiation combined with fetal bovine serum ( FBS ) in vitro, although it could not initiate the differentiation of NS/ PCs by itself. Moreover, PCA was able to induce neu-rnronal maturation and efficiently promote neurite outgrowth. On the other hand, PCA facilitated the survival of phenotypes differentiated from cultured NS/PCs, which was associated with an increased percentage of the cellular viability and a decreased percentage of cells undergoing apoptosis under differentiation conditions. In addition, PCA-induced survival was also mediated with the activating of endogenous antioxidant enzymes. Conclusion PCA promotes the neuronal differentiation and facilitates the survival of phenotypes differentiated from cultured NS/PCs.
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