高架〇迷宫实验：昆明小鼠状态焦虑动物模型的重测信度研究

摘要

Aim To explore the test-retest reliability of elevated zero maze (EZM), as an animal model of state anxiety in Kun-ming mice. Methods The behavior of adult, male and female, Kunming mice in EZM was recorded twice, for five minutes, with a one-week inter-trial interval. The following parameters were evaluated: percentage of time on EZM open arms (Otime% ), time on EZM open arms ( Otime), time on EZM closed arms (Ctime), percentage of entries into EZM open arms (Oentries% ), entries into EZM open arms (Oentries), entries into EZM closed arms (Centries), total entries into EZM open and closed arms (Entries). Subsequently, the intraclass corre-lation coefficient ( ICC) and the kappa agreement index ( κ) were calculated for these parameters. Results ICC for Otime%(♂ ICC = 0. 753,P < 0. 01;♂ + ♀ ICC = 0. 535,P < 0. 05), Otime (♂ ICC = 0. 753, P < 0. 01; ♂ + ♀ ICC = 0. 535, P <0. 05), Ctime ( ♂ ICC = 0. 753, P < 0. 01; ♂ + ♀ ICC =0. 535, P < 0. 05), and Oentries (♂ ICC = 0. 719, P < 0. 01;♀ ICC = 0. 494, P < 0. 05; ♂ + ♀ ICC = 0. 583, P < 0. 01), Centries (♀ ICC = 0. 658, P < 0. 01; ♀ ICC = 0. 508, P <0. 05; ♂ + ♀ ICC = 0. 562, P < 0. 01), Entries ( ♂ ICC =0. 691, P < 0. 01; ♀ ICC = 0. 502, P < 0. 05; ♂ + ♀ ICC =0. 574, P < 0. 05) showed a good and fair test-retest reliability, respectively. Only κ for Otime% (♂ κ = 0. 393, P < 0. 05), Ctime (♂ κ = 0. 393, P < 0. 05), Oentries (♂ κ = 0. 308, P< 0. 05; ♂ + ♀ κ = 0. 256, P < 0. 05), Centries ( ♂ κ =0. 427, P < 0. 01; ♂ + ♀ κ = 0. 238, P < 0. 05), Entries (♂κ = 0. 469, P < 0. 01) displayed a significant but poor reliability between the test-retest trials. Conclusions The parameters Otime% and Entries for Kunming mice in EZM are stable over time, and indicate an acceptable reliability with a one-week in-ter-trial interval. Meanwhile, more attention should be paid not to repeated measure effect, but to the sex effect (Male animals recommended to EZM, especially).%目的：探讨高架〇迷宫实验(elevated zero maze,EZM)作为昆明小鼠状态焦虑动物模型的重测信度。方法昆明小鼠(4周龄,♂/♀)进行 EZM 实验,实验间隔1周进行2次,采用摄像系统记录5 min 行为变化,包括两臂区进入时间及进入次数；纳入实验参数有：开臂区进入时间百分率(Otime%)、开臂区进入时间( Otime)、闭臂区进入时间(Ctime)、开臂区进入次数百分率(Oentries%)、开臂区进入次数(Oentries)、闭臂区进入次数(Centries)及两臂区进入总次数(Entries)；通过组内相关系数(intraclass correlation coef-ficient,ICC)和 kappa 一致性系数(kappa agreement index,κ)对 EZM 进行重测信度检验,并分析性别、重复测量等因素对EZM 行为模式的影响。结果 ICC 结果显示,Otime%(♂ICC =0.753,P <0.01；♂+♀ ICC =0．535,P <0．05)、Otime (♂ ICC =0．753,P <0．01；♂+♀ ICC =0．535,P <0．05)、Ctime(♂ ICC =0．753,P <0．01；♂+♀ ICC =0．535,P <0．05)、Oentries(♂ ICC =0．719,P <0．01；♀ ICC =0．494,P<0．05；♂+♀ ICC =0．583,P <0．01)、Centries(♀ ICC =0．658,P <0．01；♀ ICC =0．508,P <0．05；♂+♀ ICC =0．562,P <0．01)、Entries(♂ ICC =0．691,P <0．01；♀ ICC =0．502,P <0．05；♂+♀ ICC =0．574,P <0．01)初测重测信度相对较好,且有统计学意义；κ结果显示,Otime%(♂κ=0．393,P <0．05)、Ctime (♂κ=0．393,P <0．05)、Oentries (♂κ=0．308,P <0．05；♂+♀κ=0．256,P <0．05)、Cen-tries(♂κ=0．427,P <0．01；♂+♀κ=0．238,P <0．05)、Entries(♂κ=0．469,P <0．01)有统计学意义,但初测重测信度相对较差。结论 Otime%、Entries 作为 EZM 评价状态焦虑动物模型的稳定参数,具有尚可接受的重测信度；尤其应注意♂/♀(强烈推荐♂)对 EZM 重测信度影响效应相对较大,而重复测量影响效应相对较小。