OBJECTIVE:To control the quality of Duhuo Jishengan Koufuye.The TLC identification method of Angelica pubescens,Panax ginseng and Cinnamomum cassia and the quantitative determination of columbianetin acetate by TLCS were established. METHODS:Samples were identified respectively by TLC in contrast with Angelica Pubescens,Panax ginseng and Cinnamoum cassia drugs after treated.Columbianetin acetate in Duhuo Jishengan Koufuye was extracted refluxenthy with CHCl3 and determined by dual wavelength thin layer chromatography scanning. RESULTS:The TLC identification was highly specific and the spots were clear and concentrated.The average recovery of columbianetin acetate in samples was 96.5%(n=5).with RSD 2.44%. CONCLUSION:The quantitative method was simple,sensitive,reproducible and accurate.This quality standard was capable to effectively control real quality of Duhuo Jishengan Koufuye.%目的:为控制独活寄生口服液的质量,建立独活、人参、肉桂的薄层色谱鉴别及二氢欧山芹醇乙酸酯的薄层扫描定量方法。方法:样品中的独活、人参及肉桂进行薄层色谱鉴别。对独活中的二氢欧山芹醇乙酸酯进行薄层扫描定量。结果:鉴别方法专属性强,定量方法简便、准确,平均回收率为96.5%,RSD为2.44%。结论:本质量标准可有效地控制独活寄生口服液的质量。
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