猪苓菌酯酶同工酶及过氧化物同工酶的研究

摘要

OBJECTIVE:To study the change regular of esterase isozyme and peroxidase isozyme of Polyporus umbellatus. METHOD:Using polypropylene amide circle electrophoresis.RESULTS：The pattern and activity of isozyme in same strain had some difference at different culture days,but some of isozyme pattern bands of the Rf value were visible in whole culture stage.Every strain had its peculiar pattern bands.CONCLUSION:The number of isozyme pattern bands and enzyme activity were proportional to the growing rate of myceliun,as well as its metabolic activity.%目的：研究不同来源猪苓菌株的酯酶同工酶及过氧化物同工酶图谱的变化规律。方法：采用聚丙烯酰胺圆盘电泳法。结果：同一猪苓菌株在不同培养天数同工酶谱带及活性不相同，但是具有一定数量迁移率(Rf)相同的谱带贯穿于整个培养时间，不同菌株之间具有特征性谱带。结论：同工酶谱带的多少及活性与菌丝的生长速度即代谢的强弱成正相关。