微弧氧化镁合金对兔骨骼肌细胞黏附和增殖的影响

摘要

Objective To investigate the influence magnesium alloy modified by microarc oxidation (MAO) on the adhesion and proliferation of rabbit skeletal muscle cells. Methods The experiment was conducted in 3 groups: control group (titanium alloy), magnesium alloy group (magnesium alloy AZ31B), and MAO group (magnesium alloy AZ31B modified by MAO). The cell adhesion rates were calculated after culturing the rabbit skeletal muscle cells on alloys directly. Then the cells were cultured with the extracts, the cell relative proliferation rates were calculated, and the cell apoptosis was detected by flow cytometry. Results Compared with control group and magnesium alloy group, the cell adhesion rates in MAO group were higher at 2, 6, 24 h after direct contact culture (P <0.05), and the cell adhesion rates increased gradually as time-dependent (P <0.05). Compared with magnesium alloy group, the cell relative proliferation rates in MAO group were higher at 1, 3, 5, 7 d after extracts culture (P<0.05); the toxicity rating (grade 1) was lower than that in magnesium alloy group (grade 2). The decrease of cell relative proliferation rates was time-dependent in the magnesium alloy group (P <0.05), but in MAO group, the decrease was not time-dependent (P >0.05), and no significant apoptosis were detected in MAO group. Conclusion Magnesium alloy AZ31B modified by MAO has a good cell biocompatibility with rabbit skeletal, which can promote cell adhesion in the early stage, and has no significant effects on cell proliferation.%　　目的观察微弧氧化(MAO)镁合金对兔骨骼肌细胞黏附和增殖的影响.方法实验分为对照组(钛合金)、镁合金组(镁合金AZ31B)和MAO组(MAO镁合金AZ31B)；将兔骨骼肌细胞与材料试件直接接触培养,计算细胞黏附率；浸提液培养细胞,计算细胞相对增殖率；流式细胞仪检测细胞凋亡情况.结果直接接触培养2、6、24 h,MAO组细胞黏附率高于对照组和镁合金组(P <0.05),细胞黏附率呈时间依赖性增加(P <0.05).浸提液培养1、3、5、7 d,MAO组细胞相对增殖率较镁合金组高(P<0.05)；毒性评级为1级,低于镁合金组的2级毒性.镁合金组细胞相对增殖率呈时间依赖性下降(P<0.05),而不同时相点的MAO组细胞相对增殖率比较,差异无统计学意义(P>0.05).MAO组未见明显细胞凋亡.结论MAO镁合金可促进兔骨骼肌细胞的早期黏附,对细胞增殖无明显影响,具有良好的生物相容性.