短葶山麦冬皂苷DT-13通过抑制MMP-2/9抑制肺癌A549细胞粘附和侵袭的作用

摘要

AIM:To present the anti-tumor metastatic activity of the saponin derivative DT-13 on the A549 cell line.METHODS:The effective concentration of DT-13 was determined by the MTT assay; the ability of DT-13 on anti-adhesion was detected with the assistance of HUVECs and fibronectin; the ability of DT-13 on anti-invasion was detected by the transwell plate method; the expression of MMP-2 and MMP-9 was determined by Western blotting.RESULTS and CONCLUSIONS:DT-13 could inhibit the proliferation and adhesion of A549 cells to HUVECs and fibronectin,and the invasion of A549 cells through the extracellular matrix.In addition,DT-13 could inhibit the expression of MMP-2 and MMP-9 in A549 cells.%目的:检测短葶山麦冬皂苷DT-13对肺癌A549细胞粘附及侵袭的作用,及对金属基质蛋白表达的影响.方法:利用MTT法寻找到DT-13合适的作用浓度;利用A549细胞与人脐静脉内皮细胞、纤连蛋白的粘附,评价DT-13对细胞粘附的作用；利用transwell小室,考察DT-13对细胞侵袭的作用；采用Western Blotting方法,检测DT-13对A549细胞中金属基质蛋白-2/9的作用；结论:DT-13在10和30μmol·L-1时,可显著地抑制A549细胞的粘附与侵袭,并且对A549细胞中金属基质蛋白-2和金属基质蛋白-9的表达均有抑制作用.