大高良姜根茎中的两个新苯丙素化合物

摘要

AIM:To study the chemical constituents and bioactivity of the rhizomes of Alpinia galangal Willd.(Zingiberaceae).METHODS:The chemical constituents were isolated using various chromatographic methods and crystallization,and the chemical structures were elucidated on the basis of spectral analysis.In addition,cytotoxic activities toward four tumor cell lines (HepG2,EC109,A549 and Vero) were tested by MTT method.RESULTS:Six phenylpropanoids were obtained and their structures were identified as (S)-l'-ethoxy chavicol acetate (1),(E)-4-acetoxy cinnamyl ethyl ether (2),(E)-4-hydroxycinnamaldehyde (3),(E)-4-acetoxy cinnamyl alcohol (4),4-acetoxy cinnamyl acetate (5),and 4,4'[(2E,2'E)-bis(prop-2-ene)-1,1'-oxy]-diphenyl-7,7'-diacetate (6).CONCLUSION:Compounds 1 and 2 were two new phenylpropanoids.Compound 5 shows selective cytotoxic activity on human lung adenocarcinoma cell A549 (IC50 19.35 μmol·L-1).%目的:研究大高良姜的化学成分及活性.方法:运用各种柱色谱方法和重结晶手段分离纯化化合物,并通过波谱数据鉴定化合物结构.此外,运用MTT法测定了化合物对四种肿瘤细胞株(HepG2,EC109,A549和Vero)的细胞毒活性.结果:从大高良姜中分离得到6个苯丙素类化合物,其结构分别鉴定为(S)-1'-ethoxy chavicol acetate (1),(E)-4-acetoxy cinnamyl ethyl ether (2),(E)-4-hydroxy-cinnamaldehyde (3),(E)-4-acetoxy cinnamyl alcohol (4),4-acetoxy cinnamyl acetate (5)和4,4'[(2E,2'E)-bis(prop-2-ene)-1,1'-oxy]-diphenyl-7,7'-diacetate (6).结论:化合物1和2为新化合物.化合物5对A549肿瘤细胞有一定的细胞毒活性,IC50为19.35 μmol·L-1.