SGK1调节 Th17细胞分化在高血压中的作用

摘要

Objective To study the expression of SGK1 in CD4+ T from peripheral blood mononuclear cells (PBMC) of hypertension patients, and to explore the function of SGK1 in hypertension by regulating Th17 cells differentiation. Methods The patients with hypertension were recruited and divided into hypertension I, hypertension II and hypertension III according to clinical diagnosis of hypertension. The percentage of Th17 cells were analyzed by FCM. ELISA was using to analyze the level of IL-17A in patients’ serum with hypertension. The CD4+ T cells were prepared from PBMC of hypertension patients by using magnetic beads. The expression of SGK1 and RORC were detected by real-time PCR. The hypertension animal models were constructed by high salt diet. The inflammation form kidney was observed by HE staining, and SGK1 expression in kidney was detected by IHC. Results Compared with the healthy control, the percentage of Th17 cells and the level of IL-17A in serum were increased significantly in patients with hypertension. Importantly, the levels were higher along with the development of hypertension. SGK1 expression was up-regulated remarkably in CD4+ T cells from patients with hypertension. What’s more, SGK1 expression has positive correlation with RORC and IL-17A. In the hypertension animal models, compare with control, the levels of serum IL-17A was increased, the inflammation were observed in hypertension animal models and the expression of SGK1 in kidney was increased significantly. Conclusion The percentage of Th17 cells were increased in patients with hypertension, and SGK1 promoted the progress of hypertension by regulating the differentiated of Th17 cells.%目的：探讨 SGK1在高血压外周血 CD4+T 淋巴细胞中的表达，揭示 SGK1通过调节 Th17细胞的功能在高血压中的作用。方法收集高血压患者病例，依据临床诊断分成 I 级、II 级和 III 级。FCM 检测 Th17细胞在高血压患者外周血中的比例，ELISA 检测 IL-17A 在血清中的表达水平。从高血压患者外周血单个核细胞中分选 CD4+T 淋巴细胞， real-time PCR 检测 SGK1的表达，同时检测 RORC 的表达水平。构建高血压大鼠模型，HE 染色观察肾组织炎症变化，免疫组织化学检测肾组织中 SGK1的水平。结果与健康对照相比，高血压患者外周血 Th17细胞的比例和血清中 IL-17A 的水平明显升高，且随高血压分级越高，Th17细胞比例和 IL-17A 血清水平越高。SGK1的表达在高血压患者 CD4+T 淋巴细胞中显著升高。更为重要的是，SGK1的表达与 RORC 和 IL-17A 具有正相关性。在高盐诱导的高血压大鼠模型中，血清 IL-17A 的水平明显高于正常对照组。与对照组比较，高血压组的肾组织出现了明显的炎症变化和浸润的淋巴细胞，SGK1在肾组织中的表达明显升高。结论高血压中 Th17细胞的比例明显升高，SGK1通过调节 Th17细胞的功能促进了高血压的进展。