Modified Approach for Establishing Filament-induced Permanent Middle Cerebral Artery Occlusion Rats and Mice Models

摘要

BRAIN ischemia stroke is a devastating disease,with more than 10％ stroke patients either severely disabled or dead.Although rodent filament middle cerebral artery occlusion (MCAO)model can mimic human brain ischemic stroke well,its wide use was restricted by difficulty in surgery,especially in ligating pterygopalatineartery (PPA).Twenty-seven male Sprague-Dawley rats were randomly divided into A,B,C groups (n=9).All the rats were performed MCAO surgery with sutures of difference sizes.Group A used 4-0 blocking suture plus ipsilateral PPA block,group B used 3-0 blocking suture plus ipsilateral common carotid artery (CCA) block,and group C used 3-0 suture plus ipsilateral PPA block.Twenty-seven male C57BL/6 wild type mice were also randomly divided into a,b,c groups.Group a used 5-0blocking suture plus ipsilateral PPA block,group b used 6-0blocking suture plus ipsilateral CCA block,group c use 6-0suture plus ipsilateral PPA block.