端粒酶、端粒酶RNA组分和端粒酶催化亚单位在肺癌中的表达

摘要

Objective To investigate whether telomerase activity, human telomerase RNA (HTR) and human telomerase reverse transcriptase (HTERT) expression were associated with tumor development in lung cancer and whether telomerase is regulated at gene level or transcriptional level.Methods Expression of HTR and HTERT was detected by reverse transcription-polymerase chain reaction (RT-PCR) in 68 human lung cancer and in 68 adjacent-neoplatic lung tissues. And telomerase activity was examined by a quantitative telomeric repeat amplification protocol (TRAP).Results In 68 lung cancer tissues, telomerase activity, HTR and HTERT were expressed in 79%, 98.5% and 91.2% respectively, whereas all adjacent non-neoplastic lung tissues were telomerase negative. Most normal lung tissues expressed HTR (91.2%) and HTERT was detected in only 7 of 68 non-neoplastic tissues.Conclusions The relatively high frequency of telomerase activity in lung cancer whereas the detection of no telomerase activity in normal lung tissues suggested that telomerase may play an important role in tumorigenesis of lung cancer. Compared to HTR, HTERT expression was better associated with telomerase activity with a concordance of 88.9%. Telomerase activity may be regulated at transcription level or translation level.%目的　探讨hTR 、hTERT与端粒酶活性的表达是否与肺癌的发生发展有关，深入了解端粒酶基因对端粒酶活性的调控是在基因水平还是在转录水平。rn方法　采用TRAP-PCR和RT-PCR方法分别检测68例肺癌组织、68例癌旁组织端粒酶活性、端粒酶基因hTR和 hTERT 的表达。rn结果　68例肺癌组织中端粒酶阳性率为79%，HTR阳性率为98.5%，HTERT阳性率为91.2%。68例癌旁组织中，无一例表达端粒酶阳性，且大多数癌旁组织均表达HTR（91.2%），而HTERT在68例癌旁组织中仅7例为阳性。rn结论　肺癌组织中表达高频率的端粒酶活性而癌旁组织无一例表达端粒酶阳性说明了端粒酶的活性是肺癌发生发展所必须的。与HTR相比，HTERT同端粒酶具有更高的一致性，其一致率为88.9%。HTR与HTERT可能是在转录后或翻译后水平对端粒酶进行调控的。