UF-1000i全自动尿有形成分分析仪对血尿检测的携带污染分析

摘要

Objective To investigate the interference of blood urine on red cell detection of subsequent adjacent specimen on Sysmex UF-1000i flow cytometer urine sediment analyzer and to propose some countermeasures.Methods Totally 60 copies of fresh urine specimens having the results in normal range were chosen,and then each copy was divided into three pieces.All the pieces of specimens were put on the tube rack between the low-,medium-and high-concentration blood urine,and the urine was in front of the specimens.UF-1000i automatic urinary sediment analyzer was used for detection under auto sampling mode,and paired t-test was carried out for the results before and after interferences by concentrations of blood urine.The carryover was measured for the specimens and blood urine,and the specimens underwent microscopy.Results The RBC results had no significant difference before and after the interference by low-concentration blood urine (P＞0.05),while had statistical differences before and after the interferences by medium-and high-concentration blood urine (P＜0.01).There was also obvious difference between the RBC results interfered by medium-concentration blood urine and by highconcentration one.There were no significant differences between the results of WBC,epithelial cell and cast before and after the interferences (P ＞0.05).The urinary sediment microscopy results were within the normal range,and the highconcentration blood urine had the carryover gain the maximum value.Conclusion UF-1000i automatic urinary sediment analyzer has the RBC result higher than the actual one after the interference,and the interference deteriorates with the increased concentration of blood urine.The blood urine specimen has to be tested separately,and deep cleaning has to be performed for the analyzer.The test by the analyzer had better be combined with microscopy to avoid carryover.%目的:研究UF-1000i全自动尿有形成分分析仪对血尿的检测是否有携带污染以及如何避免.方法:从门急诊患者中挑选60份结果在正常范围内的新鲜尿液标本进行常规检验.每份取3管,分别与低、中、高3种浓度的血尿间隔放置在试管架上(血尿标本在前),用UF-1000i全自动尿有形成分分析仪自动进样模式检测.对3种浓度血尿干扰前后结果进行配对t检验,比较各项结果有无差异,同时测定各自的携带污染率,并对干扰后尿液进行显微镜镜检.结果:低浓度血尿干扰后红细胞与干扰前结果无明显差异(P＞0.05),中浓度血尿与高浓度血尿干扰后红细胞结果与干扰前有明显差异,具有统计学意义(P＜0.01),且中浓度与高浓度血尿干扰后结果之间的差异也具有统计学意义(P＜0.01);白细胞、上皮细胞、管型与干扰前均无明显差异(P＞0.05).尿沉渣镜检结果在干扰后均在正常范围内,携带污染率上高浓度血尿干扰最大.结论:血尿致UF-1000i全自动尿有形成分分析仪下一个标本红细胞检测结果较真实结果偏高,且随血尿浓度的增加,干扰程度也变大.对于血尿样本,应注意单独检测,并对仪器进行深度冲洗,与显微镜镜检联合应用,尽量避免携带污染,以保证下一标本的准确检测.