目的研究幽门螺杆菌L型(Hp-L型)和HPV16、18感染与人食管癌的关系，探讨Hp-L型致癌机制。方法应用革兰染色和免疫组化染色技术，对112例食管鳞癌和30例对照组进行Hp-L型和HPV16、18同步检测。结果革兰染色L型检出率(67.9％)与对照组(26.7％)有显著性差异(P＜0.05)；与免疫组化Hp-L型抗原检出阳性率(65.2％)无显著性差异(P＞0.05)。Hp-L型检出阳性事为61.6％(69／112)。HPV16、18表达阳性率为69.6％，与Hp-L型检出阳性率无显著性差异(P＞0.05)；Hp-L型和HPV16、18同时阳性者占59.8％(67／112)结论 Hp-L型和HPV16、18感染与食管癌发生相关。Hp-L型感染可能是食管癌除病毒之外的又一生物性致癌因素。%To study the association of Helicobacter pylori L-form(Hp-L) infection and HPV16、18 infection and human esophageal carcinoma (EC). To study the role of Hp-L in EC. Methods : Gram's staining and immunohistochemical techniques was used to detect the Hp-L in fection and the expression of HPV16、18 protein in 112 cases of EC with 30 normal esophageal squamous epithelial. Results The positivity rate of the infection of bacterial L-form(67. 9％ )in cancer group were significantly higher than that in normal group(26. 7％)with Gram's staining (P＜0. 05). The antigen of Hp-L in 65. 2％ cases was detected by immunohistochemical technique. The results showed no differences in both stainiing methods (P＞0. 05). The positivity rate of the Hp-L infection was 61.6％(69/112) in 112 EC. The expression of HPV16、18 were significantly higher in cancer group than that in normal group (P＜0. 05). The positive obtained in both detecting (Hp-L positive detecting and HPV16、18 positive expressing) were 59. 8％ (67/112) in cancer group. Conclusion Hp-L infection and HPV16、18 infection was closely related with EC.Hp-L infection may be one of the biological factor leading to the EC except virus.
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