福建省2004-2010年登革病毒E蛋白基因序列分析

摘要

Since several dengue viruses (DENV) have been isolated in Fujian Province during the past decade, sequencing and evolution analyses of viral envelope genes are helpful in determining their possible transmission origins. In this study, viral RNA was extracted from 12 DENV strains from Fujian between 20042010. Viral envelope genes were amplified, cloned into TA vectors and sequenced, and the sequence data were subsequently analyzed by bioinformatics software. Full-length E genes of DENV-1 or DENV-2 of 1 485 bp, and DENV- 3 of 1 479 bp were obtained. It was indicated, from BLAST analysis and phylogenetic trees, that DENV strains in Fujian Province during 20042010 shared the highest similarity with Southeast Asian strains, suggesting that DENV circulating in Fujian Province between 20042010 were probably imported from Southeast Asia. Hence, extensive monitoring on passengers from this region at the entry-ports should be strengthened.%通过登革病毒包膜蛋白基因序列测定与进化分析,确定20042010年间福建省登革病毒的主要来源地.方法 提取登革病毒分离株的RNA,扩增病毒外膜蛋白基因,扩增产物经TA克隆后提取质粒并测序,应用相关专业软件做序列分析.结果 在测定的12株登革病毒外膜蛋白基因核酸序列中,1、2型病毒的序列长度均为1 485 bp,3型为1 479 bp.BLAST比对与序列进化树均表明,2004-2010年期间,福建省所分离的登革病毒分离株与东南亚一带流行的毒株一致性最高,福建省与东南亚流行的毒株间具有高度的同源性.结论 2004-2010年福建省流行的登革病毒应当是由东南亚一带输入,应加强对该地区入境人员的监测工作.