布氏菌苗皮内与划痕接种的效果评价

摘要

ABSTRACT:The guinea pigs were immunized by the Brucella vaccine through intradermal injections and the skin scratch respectively ,and then the immune effects of the two ways were evaluated .Serum samples were collected one month after the last injection and detected for the total IgG titer by interval ELISA .Cell‐mediated immune was evaluated by late‐onset hyper‐sensitivity .The guinea pigs were challenged with Brucella melitensis M5 ,and then were killed to isolated M5 from spleen of each guinea pig to compare the protective effects of two methods of immunization .The ELISA results showed that both of the two methods of immunization could induce strong humoral immune response ,and DTH response to Br‐PPD antigen were 100%in both methods .No significant difference in the immune protective effect of two methods was detected .Results of humoral im‐munity ,cellular immunity and protective effect showed the same effect by intradermal injections and skin scratches .%目的：将布氏疫苗通过皮内注射和皮上划痕方式免疫豚鼠，比较两种方式的免疫效果，为进一步研究人用皮内注射布氏菌活疫苗奠定基础。方法采用两种免疫方法免疫动物，免疫一个月后进行采血，用ELISA法检测血清中总IgG抗体；通过皮肤迟发性超敏反应进行体内细胞免疫评价；用羊布鲁氏菌弱毒株M5攻击免疫动物，通过脾脏细菌计数评价并比较两种免疫方法的保护效果。结果抗体检测结果显示，两种免疫方法均能诱导较强的体液免疫应答；皮内注射和皮上划痕对Br‐PPD抗原DT H阳转率均为100％；两种免疫方法的保护效果差异无统计学意义。结论通过体液免疫检测、细胞免疫评价和疫苗免疫保护力3个方面结果显示，皮内注射和划痕接种两种途径免疫效果相当。