噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠的研究

摘要

We explored the effect of therapy with bacteriophages for pandrug resistant Acinetobacter baumannii infections.Kunming mice (Specific Pathogen Free) were divided into two groups:experimental group (severe infection caused by pandrug resistant Acinetobacter baumannii) and the control group.The mice of experimental group were treated with Phages AB46 while the mice of control group were treated with broth.The survival rate was compared with statistical method and the spleen bacteria count was analyzed.Results showed that there were statistical difference between the experimental group and control group of Pandrug resistant Acinetobacter baumannii groups of 1 ∶ 10 dilution,P=0.020＜0.05.There were no statistical difference between each two groups of 1 ∶ 2 (P=0.650) and 1 ∶ 5 (P=0.170) dilution,both were＞0.05.There were no dead mice in groups of 1 ∶ 50 dilution with statistical difference of spleen bacteria count,P=0.026.Therapy with phages was an ef fective method to control infection caused by Pandrug resistant Acinetobacter baumannii which could increase the survival rate and decrease spleen bacteria count of the mice with light infection.%目的 探讨噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠的疗效.方法 以SPF级昆明小鼠建立小鼠感染泛耐药鲍氏不动杆菌的模型,分为实验组和对照组,实验组使用噬菌体AB46治疗,对照组给同等体积的肉汤治疗,统计比较两组小鼠的生存率,进行脾菌数分析.结果 泛耐药鲍氏不动杆菌1∶10稀释实验组与对照组P=0.020＜0.05,有统计学差异;1∶2、1∶5稀释组分别为P=0.650,P=0.170,均＞0.05,无统计学差异;1∶50稀释的实验组与对照组均无小鼠死亡,脾菌数计数结果P=0.026,有统计学差异.结论 噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠是一种有效的治疗方法,可明显提高小鼠的生存率,在感染较轻的情况下可以明显降低小鼠的脾菌数.