含噻唑环的磺胺母核合成与鉴定

摘要

The sulfathiazole derivative N^1 - [ 4 - （ carboxymethyl ） - 2 - thiazolyl ] sulfanilamide （ TS ） was synthesized by （2 - amino - 4 - thiazolyl ） - acetic acid （ATAA） and N - acetyl sulfanilyl chloride （ ASC ）. The three - step chemical reactions of esterlysis, affinity - replace reaction and ester hydrolysis were accomplished. Coupling of TS to Bovine Serum Albumin （BSA） formed the complete antigen （ TS - BSA） by EDC method. TS was confirmed by mass - spectrum ： [ M - ] = 311.92, corresponding with theoretical value [ M ] = 313.01. The conjugates was identified by UV - scanning. The characteristic peak of UV spectra of TS - BSA was shifted and the conjugates was confirmed. The coupling ratio was 11 ： 1.%详细介绍了以2-氨基-4-噻唑乙酸（ATAA）和对乙酰氨基苯磺酰氯（ASC）为原料，经过酯化、亲和取代、酯的水解反应合成含噻唑环的磺胺母核即N^1-[4-（羧甲基）-2-噻唑]对氨基苯磺酰胺（TS）的过程及采用碳二亚胺法（EDC法）合成完全抗原TS-牛血清白蛋白（TS-BSA）的方法。应用质谱法鉴定TS，紫外扫描鉴定完全抗原TS-BSA。质谱结果显示[M^-]：311．92，与理论值[M]=313．01相符，表明Ts合成成功；紫外扫描特征峰值发生偏移，证明完全抗原偶联成功，计算其结合比为11：1。