鸡传染性贫血病毒VP 1、VP 2基因的表达及免疫原性研究

摘要

The VP1 and VP2 genes of chicken anemia virus were cloned into pFastBacHTA vectors separately, then transformed into competent cells of E. coli DH10Bac. The recombinant baculoviruses vBac-VP1 and vBac-VP2were generated by transfection of recombinant bacmid into Sf21 insect cells. Finally, recombinant proteins were expressed in suspension culture Sf21 cells. SDS-PAGE and Western blotting showed that VP1 and VP2 have been expressed in Sf21 cells. Immunized the SPF chicken with recombinant protein, their serum were inspected by ELISA and showed good immunogenicity.%为探索鸡传染性贫血重组蛋白亚单位疫苗的可行性，将鸡传染性贫血病毒基因分别克隆到转移载体pFastBacHTA中，再将其分别转化DH10Bac感受态细胞得到相应的重组表达载体，转染昆虫细胞Sf21获得含有VP1、VP2基因的重组杆状病毒vBac－VP1、vBac－VP2，应用悬浮培养的Sf21细胞表达重组蛋白。 SDS－PAGE及Western blotting结果证明， VP1、VP2基因在昆虫细胞中得到了表达，动物试验进一步证实，重组蛋白免疫SPF鸡可产生ELISA抗体，提示杆状病毒表达的VP 1、VP 2具有良好的免疫原性。