剑麻根际联合固氮菌的分离及固氮活性测定

摘要

通过选用NFM、CCM、Ashby及改良的Dobereiner培养基,利用气相色谱仪(GC)对海南省昌江县、广西扶绥县和广东雷州市三地剑麻种植基地的剑麻根际联合固氮菌进行分离、纯化及利用乙炔还原法进行固氮活性测定,获得50株具有固氮活性的菌株,其固氮酶活性最高的菌株是ASN004,固氮酶活性为1 765.659 0 nmol/(mL·h),具有开发利用的潜力.%The associative nitrogen fixation bacteria in sisal rhizosphere, sampled from Changjiang County in Hainan Province, Fusui County in Guangxi Zhuang Autonomous Region and Leizhou City in Guangdong Province, were isolated and purified by Gas Chromatography on the NFM, CCM, Ashby and modified Dobereiner medium. And their nitrogen -fixing activity was measured by acetylene reduction method. The result indicated that, 50 bacterial strains had nitrogen -fixing activity. And a bacterial strain No. ASN004 with the highest nitrogenase activity of 1765.6590 nmol/(mL·h) was found and it had good exploitation potential.