银合欢AFLP分子标记体系的优化

摘要

DNA was extracted from the materials of Leucaena seedlings with CTAB and kit. The suitable AFLP molecular marker system was established for Leucaena diversity analysis after AFLP reaction had been optimized with DNA samples in different digestion time and primers screening. This work provided a basic knowledge on AFLP molecular marker for studying Leucaena molecular biology and molecular breeding.%以银合欢幼苗为材料,采用CTAB区室法和试剂盒法2种方法提取DNA,通过对影响AFLP反应关键因子,如DNA样品酶切时间、引物等进行优化,建立了适合银合欢AFLP分子标记的反应体系,为利用AFLP标记对银合欢进行分子生物学研究及分子育种奠定基础.