香蕉枯萎病菌4号生理小种致病性突变体△Focr4-1422生物学特性研究

摘要

为研究香蕉枯萎病菌4号生理小种致病性突变体相关致病基因的功能,研究了基因敲除子△Focr4-1422与野生型菌株193之间部分致病性相关的表型差异.结果表明,基因敲除子对巴西蕉的致病性减弱,荧光检测显示菌丝能够从根部扩散到根茎交界处的茎部.△Focr4-1422对细胞壁降解酶的抗性大大增强.基因敲除子△Focr4-1422和野生型193在相同的温度、pH值、光照条件和碳氮源培养基下的生长速率均没有达到极显著差异,两者的菌丝和孢子的致死温度也一致.%In order to identify the functions of the pathogenicity —related genes of Fusarium oxysporum f.sp. cubense (Foe) race 4, the phenotypic differences of pathogenic relevance between genes knockout sub ΔFocr4-1422 and wild type strain 193 were studied. Results revealed that ΔFocr4—1422 lost the pathogenicity to the Brazil banana; Fluorescence detection showed hyphae could spread to the junction of the stems from the root; ΔFocr4-1422 had greater resistance to cell wall degrading enzymes; the growth rate of ΔFocr4-1422 and wild typel93 was not significant at the same temperature, pH, light conditions and in the same carbon and nitrogen source medium. The lethal temperature of both mycelia and spores was the same.