实时荧光定量PCR检测木薯海藻糖合成酶基因(MeTPS1-3)干旱胁迫下的表达

摘要

采用实时荧光定量PCR的方法测定木薯SC124和KU50两个品种在模拟干旱胁迫下海藻糖合成酶基因(Me TPS1-3)的转录水平变化.结果显示,与正常水分组相比:干旱处理2h后,SC124根、茎、下部成熟叶和上部幼叶MeTPS1-3基因的表达量没有明显变化；而在KU50中,根部相对表达量上升了12倍,茎、下部成熟叶和上部幼叶没有明显变化.干旱处理24 h后,SC124根部相对表达量上升约30倍、茎部有微小的上升、下部成熟叶片却有小幅下降、上部幼叶部位上升约4倍；在KU50品种中,根部上升约14倍,茎部有微小的下调,下部成熟叶和上部幼叶表达量下调.木薯遭受干旱胁迫时,根部MeTPS1-3基因上调表达最明显,由此推测其在木薯抗干旱胁迫中起重要调控作用.%The differential expression of trehalose synthetase gene (MeTPS1-3) in cassava SC124 and KU50 under different simulation drought stress was analyzed by real-time fluorescence quantitative polymerase chain reaction.The results showed that the expression levels of MeTPS1-3 did not change significantly in the roots,stems,mature leaves and spire of SC124 after 2 h drought treatment.In the roots of KU50,the expression level increased about 12 times,a small increase was detected in the stems,while the level in teh spire remained unchanged.The expression levels in the roots and spire rose about 30 and 4 times,respectively,after 24h drought treatment,while a small increase in the stems and a little decrease in the mature leaves were observed.The expression level in the roots of KU50 rose about 14 times after 24 h drought treatment,while in KU50,a little drop was detected in the stems,mature leaves and spire.In conclusion,the most significant change of the expression of MeTPS1-3 under drought stress was observed in the roots of both cultivars,thus we supposed that the MeTPS1-3 gene played an important role in drought resistance of cassava.