以猫尾射无菌播种苗(去除根部)为外殖体,对其愈伤组织诱导和分化及其不定芽增殖进行研究.结果表明,外殖体在MS+6-BA 1.0mg/L+FNAA 0.1 mg/L培养基上,愈伤组织诱导和分化的效果较好,愈伤诱导率为82.0%,分化率为74.5%;经不定芽增殖培养基MS+6-BA 0.5 mg/L+NAA 0.05 mg/L培养,不定芽增殖倍数为8.2,平均株高为4.7cm.组培苗在MS+IBA 0.5mg/L培养基上生根率达90%.生根苗移栽成活率达84%.%The sterile seedlings of Uraria crinita (L.) Desv.ex Dc, with the roots removed, were used as explants for tissue culture.And the most suitable media for callus induction, differentiation and adventitious buds proliferation were studied.The results showed that the callus induction and differentiation from the explants were effective on medium MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, and the rates of the callus induction and differentiation were 82.0％ and 74.5％.The best medium for adventitious buds proliferation was MS+6-BA 0.5 mg/L +NAA 0.05 mg/L, and the multiple of adventitious buds proliferation and the average height of seedlings were 8.2 and 4.7 cm.The rooting rate of sterile seedlings on MS+IBA 0.5 mg/L was 90％.The rooted plantlets were transplanted into pots, and the rate of survival was 84％.
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