小水榕茎部内生菌的分离及鉴定

摘要

采用稀释涂板法，对小水榕茎部的内生菌进行分离、纯化及16S rDNA鉴定，通过同源性比对构建系统发育树。结果表明：分离到的24株内生菌分别属于5个类群(Alphaproteobacteria、 Gammaproteobacteria、 Firmicutes、Bacteroidetes、 Filimonas)的11个属(Phenylobacterium、 Rhizobium、 Caulobacter、 Sphingobium、 Paenibacillus、Staphylococcus、 Bacillus、 Pseudomona、 Chitinophaga sancti、 Filimonas、 Agrobacterium)，其中Alphaproteobacteria、Firmicutes为优势种群，占总数的45.8%；所有菌株与其同源菌株的相似性均在98%以上。本研究有助于揭示小水榕茎部内生菌的多样性，为发掘其应用潜力提供参考资料。%A total of 24 isolates of Endophytic bacteria from the inside of the stem of Anubias barteri was isolated by spread-plate method. These isolates were aligned by using the sequences of 16S rDNA and then the phylogenetic tree was constructed. The result showed that 24 isolates belong to 11 genus (Phenylobacterium, Rhizobium, Caulobacter, Sphingobium, Paenibacillus, Staphylococcus, Bacillus, Pseudomona, Chitinophaga sancti, Filimonas, Agrobacterium) of 5 groups (Alphaproteobacteria, Gammaproteobacteria, Firmicutes, Bacteroidetes, Filimonas). The identity of all isolates were more than 98% same as the sequence of bacteria's in NCBI and 45.8% of them were Firmicutes that were dominant species. The result was beneficial for further study of the diversity of endophytic bacteria in Anubias barteri's stem.