全身放射损伤对伤口早期局部组织P65蛋白表达及伤口液TNF-α和IL-1的含量与活性的影响

摘要

Objective To study the effect of total body irradiation on the quantity and activity of P65,TNF α and IL 1 in irradiated rat wounds. Methods Tumor necrosis factor α (TNFα ) and interleukin 1 (IL 1 ) in wound fluids were quantified by ELISA and their biological activities were measured by L929 cell lysis and thymocyte proliferation,respectively. The expression of P65 protein in wounded tissue was determined by western blotting. Results The biological activities of TNF α and IL 1 in wound fluid decreased significantly after irradiation,while their quantities in wound fluids did not decrease significantly.The expression of P65 protein in wounded tissue also did not decrease obviously after irradiation. Conclusion These results suggestted that total body irradiation may inhibit the biological activities of TNF α , IL 1 and nuclear factor B,there may exist negative factors to disturb the protein functions in wound environments.