黄芪和大青叶对病毒性心肌炎小鼠的保护作用

摘要

Objective To investigate perfect therapeutic measure of Chinese traditional herbs through observing curative effect of single dose traditional Chinese medicine on murine viral myocarditis(VMC).Method Balb/C mice were inoculated intraperitoneally with diluted solution of coxsackievirus B3(CVB3) ,and then were divided into three groups randomly:infected control group,Mongolian milkvetch root treated group;Indigowood treated group.The mice were put to death and their hearts were examined on day 3,5,7,10,14,21 post inoculated,and were stained with hemotoxylin and eosin(HE) to observe myocardial lesions and to estimate myocardial histopathological scores,simultaneously to separate virus from myocardium.In addition,we observed myocardial ultrastructure by electron microscopy on 7th day post inoculated.Result Compared with infected control group,myocardial histopathological scores of both Mongolian milkvetch root treated group from 5th day to 21st day post inoculated and Indigowood treated group on 5th day and 7th day post inoculated showed significant decrease(P< 0.05).Conclusion Mongolian milkvetch root could protect myocardial cells.