高原低氧对胎鼠海马神经元N甲基-D天冬氨酸受体发育的影响

摘要

目的 :观察高原低氧环境对胎鼠海马神经元 N 甲基-D 天(门)冬氨酸受体( N-methyl-D-aspartate receptor, NMDAR)数目和通道特性的影响 , 为防治高原低氧引起的脑损伤提供依据. 方法:将孕 10 d SD大鼠置于低压氧舱内,采用原位杂交和膜片钳观察其所生子鼠海马 NMDA受体的数量和功能. 结果:胎鼠高原低氧后, NMDA受体数量减少,通道开放概率由 0.150降为 0.012,通道开放时间常数б 1由 0.040± 0.010降为 0.020± 0.007(t=33.21,P< 0.05),б 2由 0.75± 0.23降为 0.49± 0.23(t=25.31,P< 0.05),通道关闭时间常数б 1由 0.14± 0.07升高为 14.25± 3.5(t=12.74,P< 0.01),б 2由 2.67± 1.12升高为 4832± 1809(t=8.44,P< 0.01). 结论:高原低氧影响到胎鼠 NMDA受体的发育,提示高原低氧环境下大鼠的学习记忆可能受到影响.%AIM:To observe the effects of simulated high-altitude hypoxia on N-methyl-D-aspartate receptors(NMDAR) in newborn rats,so as to provide theoretical basis for preventing and treating high-altitude hypoxia induced cerebral injury. METHODS:Ten SD rats in pregnancy for 10 d were subjected to treatment in hypoxic chamber.In situ hybridization technique and patch clamp were employed to identify the number and function properties of NMDAR in the newborn rats. CONCLUSION:High-altitude hypoxia can affect NMDAR development in newborn rats,suggesting that learning and memory capacity of rats might be impaired in high-altitude hypoxia environment.